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rabbit anti ldha  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti ldha
    Rabbit Anti Ldha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 478 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ldha/LDHA+Rabbit+mAb/pmc13015250-6-0-2
    Average 96 stars, based on 478 article reviews
    rabbit anti ldha - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Targeting Oxalate Production by Combining Enzyme Inhibition and Proteolysis Activation: A Novel Therapeutic Approach for Primary Hyperoxaluria Type 1
    Article Snippet: Protein concentration was determined using the Quick Start Bradford Dye Reagent (Bio-Rad, 5000205). .. Western blotting was performed using the following primary antibodies: rabbit anti-AGXT (Sigma, HPA035370, 1:1000), rabbit anti-LDHA (Cell Signaling Technology, 2012, 1:1000), rabbit anti-HAO1 (Abcam, 194790, 1:1000), mouse anti- β -actin (Cell Signaling Technology, 3700S, 1:1000), mouse anti-GAPDH (Santa Cruz Biotechnology, sc365062, 1:5000) and rabbit anti-ATG5 (Cell Signaling Technology, 12994S, 1:1000). .. Fluorescent secondary antibodies—donkey antirabbit (Li-Cor, 926–68073, 1:20,000) and donkey antimouse (Li-Cor, 926–32212, 1:20,000)—were used for detection with a Li-Cor Odyssey XF Imager.

    Article Title: Integration of metabolomics and transcriptomics reveals the mechanism of TMEM30A downregulation induced FSGS podocyte injury.
    Article Snippet: 17 Podocyte injury plays a critical role in the pathogenesis and progression of focal and 18 segmental glomerulosclerosis (FSGS).. Transmembrane protein 30A (TMEM30A) 19 downregulation participates in podocyte injury.. This study aimed to identify the critical 20 pathways and molecules associated with the downregulation of TMEM30A in the context of 21 FSGS podocyte injury.

    Article Title: Targeting Oxalate Production by Combining Enzyme Inhibition and Proteolysis Activation: A Novel Therapeutic Approach for Primary Hyperoxaluria Type 1.
    Article Snippet: XXXX, XXX, XXX−XXX V Protein concentration was determined using the Quick Start Bradford Dye Reagent (Bio-Rad, 5000205). .. Western blotting was performed using the following primary antibodies: rabbit anti-AGXT (Sigma, HPA035370, 1:1000), rabbit anti-LDHA (Cell Signaling Technology, 2012, 1:1000), rabbit anti-HAO1 (Abcam, 194790, 1:1000), mouse anti-β-actin (Cell Signaling Technology, 3700S, 1:1000), mouse antiGAPDH (Santa Cruz Biotechnology, sc365062, 1:5000) and rabbit anti-ATG5 (Cell Signaling Technology, 12994S, 1:1000). .. Fluorescent secondary antibodies�donkey antirabbit (Li-Cor, 926−68073, 1:20,000) and donkey antimouse (Li-Cor, 926−32212, 1:20,000)� were used for detection with a Li-Cor Odyssey XF Imager.

    Immunofluorescence:

    Article Title: Integration of metabolomics and transcriptomics reveals the mechanism of TMEM30A downregulation induced FSGS podocyte injury.
    Article Snippet: 17 Podocyte injury plays a critical role in the pathogenesis and progression of focal and 18 segmental glomerulosclerosis (FSGS).. Transmembrane protein 30A (TMEM30A) 19 downregulation participates in podocyte injury.. This study aimed to identify the critical 20 pathways and molecules associated with the downregulation of TMEM30A in the context of 21 FSGS podocyte injury.

    Staining:

    Article Title: Integration of metabolomics and transcriptomics reveals the mechanism of TMEM30A downregulation induced FSGS podocyte injury.
    Article Snippet: 17 Podocyte injury plays a critical role in the pathogenesis and progression of focal and 18 segmental glomerulosclerosis (FSGS).. Transmembrane protein 30A (TMEM30A) 19 downregulation participates in podocyte injury.. This study aimed to identify the critical 20 pathways and molecules associated with the downregulation of TMEM30A in the context of 21 FSGS podocyte injury.

    other:

    Article Title: Metabolic orchestration of NOD1 signaling by AMPK-mediated phosphorylation of ZDHHC5
    Article Snippet: Rabbit-Anti-LDHA , Cell Signaling Technology , Cat# 3582; RRID: AB_2066887.



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    A NEK8 RNA levels across biologically independent tissues from CRC in TCGA database. means ± SD, two- tailed t test, * p < 0.05, *** p < 0.001. B – C Representative NEK8 and CD8 staining in CRC tissues from our in-house cohort. Scale bar, 50 μm. B The correlation between NEK8 expression and the percentage of CD8-positive cells is shown. C Spearman’s rank correlation (two-tailed) and p-value are shown. D Kaplan–Meier analysis of overall survival in all patients with CRC according to NEK8 expression in the TCGA dataset (log-rank test P = 0.01). E – G The expression of NEK8 protein, LDHA <t>Ser196</t> phosphorylation levels and H3K18la levels were examined in 54 colorectal cancer (CRC) tissue specimens using immunohistochemistry (IHC). Representative IHC images are shown in Fig. 8E, and the statistical results are presented in Fig. 8F–G. Light blue represents the score of a single patient, while dark blue indicates identical scores from multiple patients. Scale bar, 100 μm, means ± SD, two- tailed t test. Spearman’s rank correlation (two-tailed) and p-value are shown. H A proposed model illustrating the function and mechanism of NEK8 in CRC radiosensitization and abscopal effects. Created in BioRender. Mingzhou, L. (2026) https://BioRender.com/wsrgmbf . Source data are provided as a Source data file.
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    A NEK8 RNA levels across biologically independent tissues from CRC in TCGA database. means ± SD, two- tailed t test, * p < 0.05, *** p < 0.001. B – C Representative NEK8 and CD8 staining in CRC tissues from our in-house cohort. Scale bar, 50 μm. B The correlation between NEK8 expression and the percentage of CD8-positive cells is shown. C Spearman’s rank correlation (two-tailed) and p-value are shown. D Kaplan–Meier analysis of overall survival in all patients with CRC according to NEK8 expression in the TCGA dataset (log-rank test P = 0.01). E – G The expression of NEK8 protein, LDHA <t>Ser196</t> phosphorylation levels and H3K18la levels were examined in 54 colorectal cancer (CRC) tissue specimens using immunohistochemistry (IHC). Representative IHC images are shown in Fig. 8E, and the statistical results are presented in Fig. 8F–G. Light blue represents the score of a single patient, while dark blue indicates identical scores from multiple patients. Scale bar, 100 μm, means ± SD, two- tailed t test. Spearman’s rank correlation (two-tailed) and p-value are shown. H A proposed model illustrating the function and mechanism of NEK8 in CRC radiosensitization and abscopal effects. Created in BioRender. Mingzhou, L. (2026) https://BioRender.com/wsrgmbf . Source data are provided as a Source data file.
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    A NEK8 RNA levels across biologically independent tissues from CRC in TCGA database. means ± SD, two- tailed t test, * p < 0.05, *** p < 0.001. B – C Representative NEK8 and CD8 staining in CRC tissues from our in-house cohort. Scale bar, 50 μm. B The correlation between NEK8 expression and the percentage of CD8-positive cells is shown. C Spearman’s rank correlation (two-tailed) and p-value are shown. D Kaplan–Meier analysis of overall survival in all patients with CRC according to NEK8 expression in the TCGA dataset (log-rank test P = 0.01). E – G The expression of NEK8 protein, LDHA <t>Ser196</t> phosphorylation levels and H3K18la levels were examined in 54 colorectal cancer (CRC) tissue specimens using immunohistochemistry (IHC). Representative IHC images are shown in Fig. 8E, and the statistical results are presented in Fig. 8F–G. Light blue represents the score of a single patient, while dark blue indicates identical scores from multiple patients. Scale bar, 100 μm, means ± SD, two- tailed t test. Spearman’s rank correlation (two-tailed) and p-value are shown. H A proposed model illustrating the function and mechanism of NEK8 in CRC radiosensitization and abscopal effects. Created in BioRender. Mingzhou, L. (2026) https://BioRender.com/wsrgmbf . Source data are provided as a Source data file.
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    Image Search Results


    A NEK8 RNA levels across biologically independent tissues from CRC in TCGA database. means ± SD, two- tailed t test, * p < 0.05, *** p < 0.001. B – C Representative NEK8 and CD8 staining in CRC tissues from our in-house cohort. Scale bar, 50 μm. B The correlation between NEK8 expression and the percentage of CD8-positive cells is shown. C Spearman’s rank correlation (two-tailed) and p-value are shown. D Kaplan–Meier analysis of overall survival in all patients with CRC according to NEK8 expression in the TCGA dataset (log-rank test P = 0.01). E – G The expression of NEK8 protein, LDHA Ser196 phosphorylation levels and H3K18la levels were examined in 54 colorectal cancer (CRC) tissue specimens using immunohistochemistry (IHC). Representative IHC images are shown in Fig. 8E, and the statistical results are presented in Fig. 8F–G. Light blue represents the score of a single patient, while dark blue indicates identical scores from multiple patients. Scale bar, 100 μm, means ± SD, two- tailed t test. Spearman’s rank correlation (two-tailed) and p-value are shown. H A proposed model illustrating the function and mechanism of NEK8 in CRC radiosensitization and abscopal effects. Created in BioRender. Mingzhou, L. (2026) https://BioRender.com/wsrgmbf . Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: NEK8 kinase-mediated lactate increase impairs antitumor immunity decreasing radiotherapy sensitivity in colorectal cancer

    doi: 10.1038/s41467-026-70657-z

    Figure Lengend Snippet: A NEK8 RNA levels across biologically independent tissues from CRC in TCGA database. means ± SD, two- tailed t test, * p < 0.05, *** p < 0.001. B – C Representative NEK8 and CD8 staining in CRC tissues from our in-house cohort. Scale bar, 50 μm. B The correlation between NEK8 expression and the percentage of CD8-positive cells is shown. C Spearman’s rank correlation (two-tailed) and p-value are shown. D Kaplan–Meier analysis of overall survival in all patients with CRC according to NEK8 expression in the TCGA dataset (log-rank test P = 0.01). E – G The expression of NEK8 protein, LDHA Ser196 phosphorylation levels and H3K18la levels were examined in 54 colorectal cancer (CRC) tissue specimens using immunohistochemistry (IHC). Representative IHC images are shown in Fig. 8E, and the statistical results are presented in Fig. 8F–G. Light blue represents the score of a single patient, while dark blue indicates identical scores from multiple patients. Scale bar, 100 μm, means ± SD, two- tailed t test. Spearman’s rank correlation (two-tailed) and p-value are shown. H A proposed model illustrating the function and mechanism of NEK8 in CRC radiosensitization and abscopal effects. Created in BioRender. Mingzhou, L. (2026) https://BioRender.com/wsrgmbf . Source data are provided as a Source data file.

    Article Snippet: Antibodies used were: NEK8 (Abcam, ab116721, RRID: AB_1090250, discontinued reagent); α-tubulin (Proteintech, 11224-1-AP, RRID: AB_2210206); LDHA (Proteintech, 19987-1-AP, RRID: AB_10646429); L-Lactyl Lysine(PTMBIO, PTM-1401RM, RRID: AB_2942013); H3K9la (PTMBIO, PTM-1419RM, RRID: AB_3076695); H3K14la (PTMBIO, PTM-1414RM, RRID: AB_3076697); H3K18la (PTMBIO, PTM-1427RM, RRID: AB_3076698); Histone H3 (PTMBIO, PTM-1001RM, RRID: AB_3676032); H4K5la (PTMBIO, PTM-1407RM, RRID: AB_3096309); H4K8la (PTMBIO, PTM-1415RM, RRID: AB_3101829); H4K12la (PTMBIO, PTM-1411RM, RRID: AB_2941896), H4K16la (PTMBIO, PTM-1417RM, RRID: AB_3101830); Histone H4 (PTMBIO, PTM-1015RM, RRID: AB_3101866); Anti-Rabbit IgG (Cell Signaling Technology, 7074), Anti-Rabbit LDHA (Ser196) (HUABIO, Homemade) and HLA-A (Boster, M00194-4).

    Techniques: Two Tailed Test, Staining, Expressing, Phospho-proteomics, Immunohistochemistry